Structural studies of recombinant fusion protein constructs yielded identification of a 44 amino acid domain that displayed autonomous dimerization activity and conferred a highly elongated structure, characteristic of native GRP94, to the fusion protein. In vitro translation was used to demonstrate that a C-terminal 20 kDa domain was both necessary and sufficient for dimerization. An assembly function for the C-terminal domain was established by analysis of the quaternary structure of C-terminal constructs synthesized either in vitro or through recombinant expression. Limited proteolysis resulted in the loss of approximately 16 kDa from the C-terminus and disassembly into monomers, implicating the C-terminus as the site of assembly. Velocity sedimentation and gel filtration chromatography were used to identify native GRP94 as a dimer with an extended, rod-like shape. To identify the structure/function relationships which define the molecular basis of GRP94 activity, we have performed a structural analysis of native GRP94 and identified a discrete domain, representing amino acids 676−719, which regulates dimerization and displays autonomous oligomerization activity. Send us feedback.GRP94 is an abundant, resident glycoprotein of the mammalian endoplasmic reticulum lumen and member of the hsp90 family of molecular chaperones. These example sentences are selected automatically from various online news sources to reflect current usage of the word 'chaperone.' Views expressed in the examples do not represent the opinion of Merriam-Webster or its editors. , 20 July 2019 Fleury Drive resident Cameron Drew expressed concerns about how well Black might be able to chaperone a group of teenagers.Īnnie Gentile,, 19 July 2019 Tena’s older sister, who lives near Sacramento, would drive two-and-a-half hours south to Salinas to pick her up, take her to the meetings, then chaperone her home again-eight hours of driving each time. 2019 Students came to us and asked us to chaperone them to Europe for six weeks. 2019 King was prepared to chaperone the trip and carry her daughter the whole way, but someone reached out with a suggestion.Ĭaitlin O'kane, CBS News, 24 Sep. 2019 Attendees must be 22 or younger, although parents may chaperone their teens. 2021 Some people would rather chaperone the Zombie Prom than ask for a bump in pay.Īmy Lindgren, Twin Cities, 26 Oct. 2022 The field trip has happened for a decade, but went largely unnoticed until this week, when Leonardi posted photos on social media saying she was honored to chaperone it. Verb All that changes when she is forced to chaperone her autistic sister. 2022 Sowa also didn’t have a chaperone for the appointment with that female patient.Īmanda Milkovits,, 2 Sep. Kelly Meyerhofer, Journal Sentinel, 16 Sep. 2022 For now, as the bots learn the lay of the land, a chaperone will accompany them on deliveries that involve crossing a street. 2022 Oaks Park, the venerable amusement park located in Southeast Portland, has instituted a new chaperone policy for its skating rink. 2022 In 2016, the board unanimously rejected a request from Gupta to drop the chaperone requirement because, his lawyer said, the restriction was keeping Gupta from gaining privileges at a hospital. Melissa Gomez, Los Angeles Times, 28 Sep. 2012 The theme park instituted the chaperone policy after multiple fights broke out and forced the park to close three hours early in July. 2022 According to depositions from Kappes and other students on the trip, the only other chaperone was Coleman’s friend David DeMetz, a paramedic in his mid-twenties. 2022 Starting Thursday, any chaperone who attends Knott’s Scary Farm at the Buena Park theme park with five minors will be given complimentary admission to use that same night. Noun The whole school was invited to join the ride, and about 75 students showed up, many bringing their parents along to help chaperone.
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